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物种
Human分子别名
Thrombopoietin,C-mpl ligand (ML),Megakaryocyte colony-stimulating factor,Megakaryocyte growth and development factor (MGDF),Myeloproliferative leukemia virus oncogene ligand,Accession
P40225表达序列
Ser22-Leu195, with C-terminal His Tag.表达宿主
HEK293分子量
25-33 kDa (Reducing)
纯度
>95% by SDS-PAGE, >90% by HPLC.内毒素含量
<0.1EU/μg活性
EC50 < 0.5ng/ml标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
PBS, PH7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
N Engl J Med. 1998 Sep 10;339(11):746-54.
Thrombopoietin (TPO) is a glycoprotein hormone that plays a crucial role in the regulation of platelet production and megakaryocyte differentiation. The human TPO protein consisting of 174 amino acids is primarily produced in the liver and kidneys. TPO acts by binding to its receptor, the c-Mpl receptor, which is expressed on the surface of hematopoietic stem cells and megakaryocytic progenitor cells. Upon binding, TPO activates signaling pathways that promote the proliferation and differentiation of megakaryocytes, leading to increased platelet production. TPO is essential for maintaining normal platelet levels in the blood and is a key regulator of thrombopoiesis.
生物活性
Measured in a cell proliferation assay using M-07e human megakaryocytic leukemic cells. The EC50 for this effect is less than 0.3ng/ml.
电泳
2μg (R: reducing condition, N: non-reducing condition).
体积排阻色谱(SEC-HPLC)
>90% as determined by SEC-HPLC.
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