分享产品介绍 评论(0)
物种
Human分子别名
Interleukin-12 subunit beta,IL-12B,Cytotoxic lymphocyte maturation factor 40 kDa subunit (CLMF p40),IL-12 subunit p40,NK cell stimulatory factor chain 2 (NKSF2),Accession
P29460表达序列
Ile23-Ser328表达宿主
CHO分子量
40 kDa (Reducing)
纯度
>95% by SDS-PAGE and HPLC.内毒素含量
<0.1EU/μg活性
EC50 < 10ng/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
Nat Med. 2015 Jul;21(7):719-29. Epub 2015 Jun 29.
The IL-12/IL-23 p40 monomer is a subunit shared between the interleukin-12 (IL-12) and interleukin-23 (IL-23) cytokines. It is a critical component of both the IL-12 and IL-23 heterodimers, which are proinflammatory cytokines that play essential roles in regulating immune responses. The IL-12/IL-23 p40 monomer is involved in the formation of the biologically active IL-12 and IL-23 heterodimers, which bind to their respective receptors and activate signaling pathways that modulate immune cell function. Notably, the p40 subunit itself has been shown to exhibit biological activity independent of its role in forming the heterodimers, including the ability to act as an antagonist for IL-12 and IL-23 signaling by competing for receptor binding.
生物活性
Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The EC50 for this effect is less than 10ng/ml.
电泳
2μg (R: reducing condition, N: non-reducing condition).
体积排阻色谱(SEC-HPLC)
>95% as determined by SEC-HPLC.
ELISA
Immobilized IL-12/IL-23 p40 Monomer Protein, Human (Cat. No. UA040354) at 1.0μg/mL (100μL/well) can bind IL-12 R beta 1 Fc Chimera Protein, Human with EC50 of 15.24-35.86 ng/mL.
评论(0)