分享产品介绍 评论(0)
物种
Human分子别名
Tumor necrosis factor ligand superfamily member 14; Herpes virus entry mediator ligand (HVEM-L; Herpesvirus entry mediator ligand);Accession
O43557表达序列
Asp74-Val240, with N-terminal hIgG1 Fc
表达宿主
HEK293分子量
50 kDa (Reducing)
纯度
>95% by SDS-PAGE and HPLC.内毒素含量
<0.1EU/μg活性
EC50 < 10ng/ml标记
Unconjugated标签
Huamn IgG1 Fc性状
Lyophilized Powder缓冲体系
50mM Glycine, 300mM NaCl, pH9.5.
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
·3 months, -20 to -80℃ under sterile conditions after reconstitution.
·1 week, 2 to 8℃ under sterile conditions after reconstitution.
·Please avoid repeated freeze-thaw cycles.文献引用
J Clin Invest. 2001 Dec;108(12):1771-80.
TNFSF14, also known as tumor necrosis factor ligand superfamily member 14 or LIGHT (homologous to lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes), is a cytokine belonging to the tumor necrosis factor (TNF) superfamily. TNFSF14 is a type II transmembrane protein that can be cleaved to produce a soluble form. It functions as a ligand for two receptors, herpes virus entry mediator (HVEM) and lymphotoxin beta receptor (LTβR), and is involved in regulating immune responses, inflammation, and lymphoid organ development. TNFSF14 has been implicated in various physiological and pathological processes, including the modulation of T cell activation, the regulation of immune cell migration, and the promotion of antitumor immunity.
生物活性
Measured in a cell cytotoxicity assay using HT-29 human colon adenocarcinoma cells, the IC50 for this effect is 0.58-1.7 ng/ml.
电泳
2μg (R: reducing condition, N: non-reducing condition).
反相高效液相色谱(RP-HPLC)
>95% as determined by RP-HPLC.
ELISA
Immobilized Biotinylated Human LTBR, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated plate can bind LIGHT/TNFSF14 Protein, Human (Cat. No. UA040352) with a linear range of 1.1-1.4ng/mL.
评论(0)