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物种
Human分子别名
Thrombopoietin,C-mpl ligand (ML),Megakaryocyte colony-stimulating factor,Megakaryocyte growth and development factor (MGDF),Myeloproliferative leukemia virus oncogene ligand,Accession
P40225表达序列
Ser22-Gly353 with C terminal His Tag表达宿主
HEK293分子量
80-90 kDa (Reducing)
纯度
>95% by SDS-PAGE.内毒素含量
<0.1EU/μg活性
EC50 < 0.5ng/ml标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
Nature. 1994 Jun 16;369(6481):533-8.
Blood.2002 Nov 15;100(10):3457-69.
Thrombopoietin (TPO) is a glycoprotein hormone that plays a critical role in the regulation of platelet production and megakaryopoiesis, the process by which megakaryocytes give rise to platelets. TPO acts on hematopoietic stem cells and progenitor cells in the bone marrow, stimulating their differentiation into megakaryocytes and promoting platelet formation. TPO exerts its effects by binding to the c-Mpl receptor on the surface of hematopoietic cells, leading to the activation of signaling pathways that regulate cell proliferation, survival, and differentiation. Dysregulation of TPO signaling has been implicated in various hematological disorders, such as thrombocytopenia and myeloproliferative neoplasms.
生物活性
Measured in a cell proliferation assay using M-07e human megakaryocytic leukemic cells. The EC50 for this effect is less than 1ng/ml.
电泳
2μg (R: reducing condition, N: non-reducing condition).
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