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物种
Human分子别名
R-spondin-1; Roof plate-specific spondin-1 (hRspo1);Accession
Q2MKA7表达序列
Ser21-Ala263
表达宿主
CHO分子量
27 kDa (Reducing)
纯度
>95% by SDS-PAGE.内毒素含量
<0.1EU/μg标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
PBS, PH7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
Nature. 2011 Jul 4;476(7360):293-7.
R-spondin 1 (RSPO1) is a secreted protein that belongs to the R-spondin family, which consists of four structurally related proteins (RSPO1-4) that act as ligands for the Wnt signaling pathway. RSPO1 plays a crucial role in regulating Wnt/β-catenin signaling by binding to the LGR4-6 receptors and enhancing the activity of Wnt ligands. This interaction leads to the stabilization of β-catenin and the activation of Wnt target genes involved in cell proliferation, differentiation, and stem cell maintenance. RSPO1 has been implicated in various developmental processes, including embryogenesis, tissue regeneration, and stem cell self-renewal. Dysregulation of RSPO1 signaling has been associated with several diseases, such as cancer and developmental disorders.
生物活性
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The EC50 for this effect is less than 17 ng/mL in the presence of 5ng/mL Recombinant Human Wnt‑3a.
电泳(SDS-PAGE)
2μg (R: reducing condition, N: non-reducing condition).
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