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物种
Mouse分子别名
Interleukin-31,IL-31,Accession
Q6EAL8表达序列
Thr24-Cys163, with C terminal His Tag.表达宿主
HEK293分子量
20-40 kDa (Reducing)
纯度
>95% by SDS-PAGE; >90% by HPLC.内毒素含量
<0.1EU/μg活性
ELISA: EC50 = 0.02-0.081μg/ml. Bio-Assay: EC50 < 30ng/ml.标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
PBS, PH7.4溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
Nat Immunol. 2004 Jul;5(7):752-60. Epub 2004 Jun 6.
Interleukin-31 (IL-31) is a cytokine that belongs to the IL-6 cytokine family and is primarily produced by activated T helper type 2 (Th2) cells. IL-31 signals through a heterodimeric receptor complex composed of IL-31 receptor A (IL-31RA) and oncostatin M receptor (OSMR), leading to the activation of downstream signaling pathways such as STAT1 and STAT3. IL-31 is involved in various inflammatory and immune responses, and has been implicated in the pathogenesis of allergic diseases, skin disorders, and chronic inflammatory conditions. In mouse models, IL-31 has been shown to play a role in pruritus (itching) and dermatitis, highlighting its importance in mediating inflammatory responses in the skin.
生物活性
Measured by its ability to induce STAT3 phosphorylation by U-87 cells in response to Recombinant Mouse IL-31 His Tag in the presence of 20ng/mL Recombinant Mouse IFN-γ. The EC50 for this effect is less than 30ng/ml.
电泳
2μg (R: reducing condition, N: non-reducing condition).
反相高效液相色谱(RP-HPLC)
>90% as determined by RP-HPLC.
ELISA
Measured by its binding ability in a functional ELISA. When Recombinant Human IL-31 is immobilized 2.5µg/mL (100µL/well), Recombinant Mouse IL-31RA Fc chimera binds with an EC50 of 0.02-0.081μg/ml.
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