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物种
Mouse分子别名
Thrombopoietin,C-mpl ligand (ML),Megakaryocyte colony-stimulating factor,Megakaryocyte growth and development factor (MGDF),Myeloproliferative leukemia virus oncogene ligand,Accession
P40226表达序列
Ser22-Phe196表达宿主
E.coli分子量
18 kDa (Reducing)
纯度
>95% by SDS-PAGE.内毒素含量
<0.1EU/μg活性
EC50 < 2.0ng/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
PBS, pH 7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
Annu Rev Med. 2009:60:193-206.
Thrombopoietin (TPO) is a glycoprotein hormone that plays a critical role in regulating the production and maturation of platelets in the bone marrow. TPO is primarily produced in the liver and kidney and acts on hematopoietic stem cells and megakaryocytes to stimulate platelet production. TPO binds to its receptor, the thrombopoietin receptor (c-Mpl), leading to the activation of signaling pathways that promote the proliferation and differentiation of megakaryocytic progenitor cells. TPO is essential for maintaining platelet homeostasis and is involved in various physiological processes, including hemostasis, thrombopoiesis, and immune regulation.
生物活性
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The EC50 for this effect is less than 2ng/ml.
电泳
2μg (R: reducing condition, N: non-reducing condition).
ELISA
Immobilized TPO (175aa) Protein, Mouse (Cat. No. UA040342) at 2.0μg/mL (100μL/well) can bind Thrombopoietin R/TPO R His Tag Protein, Mouse (Cat. No. UA011170) with EC50 of 0.57-0.69 μg/mL.
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