RANK L/TNFSF11(182aa) Protein, Human
Tumor necrosis factor ligand superfamily member 11 (TNFSF11),Osteoclast differentiation factor (ODF),Osteoprotegerin ligand (OPGL),Receptor activator of nuclear factor kappa-B ligand (RANKL),TNF-related activation-induced cytokine (TRANCE)
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物种
Human分子别名
Tumor necrosis factor ligand superfamily member 11 (TNFSF11); Osteoclast differentiation factor (ODF); Osteoprotegerin ligand (OPGL); Receptor activator of nuclear factor kappa-B ligand (RANKL); TNF-related activation-induced cytokine (TRANCE);Accession
O14788表达序列
Gly64-Asp245
表达宿主
CHO分子量
25-33 kDa (Reducing)
纯度
>95% by SDS-PAGE and HPLC.内毒素含量
<0.1EU/μg活性
EC50 < 6.0 ng/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20mM Tris, 100mM NaCl, pH8.0
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
Front Immunol. 2014 Oct 20:5:511. eCollection 2014.
TNFSF11, also known as RANKL (Receptor Activator of Nuclear Factor Kappa-B Ligand), is a cytokine belonging to the tumor necrosis factor (TNF) superfamily. It plays a crucial role in regulating bone metabolism and immune responses. TNFSF11 is primarily expressed by osteoblasts and activated T cells and functions as a key mediator of osteoclast differentiation and activation. By binding to its receptor RANK (Receptor Activator of Nuclear Factor Kappa-B) on osteoclast precursor cells, TNFSF11 stimulates osteoclastogenesis and bone resorption. Additionally, TNFSF11 is involved in the regulation of immune responses, lymph node development, and mammary gland development.
生物活性
Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The EC50 for this effect is less than 6ng/ml.
电泳
2μg (R: reducing condition, N: non-reducing condition).
体积排阻色谱(SEC-HPLC)
>95% as determined by SEC-HPLC.
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