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TRANCE/RANK L/TNFSF11 Protein, Mouse

Tumor necrosis factor ligand superfamily member 11 (TNFSF11),Osteoclast differentiation factor (ODF),Osteoprotegerin ligand (OPGL),Receptor activator of nuclear factor kappa-B ligand (RANKL),TNF-related activation-induced cytokine (TRANCE)

价格 ￥1,350.00 （ 1-2周）
货号 UA040335
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Mouse

  • 分子别名

    Tumor necrosis factor ligand superfamily member 11 (TNFSF11); Osteoclast differentiation factor (ODF); Osteoprotegerin ligand (OPGL); Receptor activator of nuclear factor kappa-B ligand (RANKL); TNF-related activation-induced cytokine (TRANCE);

  • Accession

    O35235

  • 表达序列

    Lys158-Asp316

  • 表达宿主

    E.coli

  • 分子量

    18 kDa (Reducing)

  • 纯度

    >95% by SDS-PAGE and HPLC.

  • 内毒素含量

    <0.1EU/μg

  • 活性

    EC50 < 3.0 ng/ml

  • 标记

    Unconjugated

  • 标签

    No Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    20mM Tris, 150mM NaCl, pH8.0

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.

    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.

    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    Front Immunol. 2014 Oct 20:5:511. eCollection 2014.

背景介绍

  • TNFSF11, also known as RANKL (Receptor Activator of Nuclear Factor Kappa-B Ligand), is a cytokine belonging to the tumor necrosis factor (TNF) superfamily. It plays a crucial role in regulating bone metabolism and immune responses. TNFSF11 is primarily expressed by osteoblasts and activated T cells and functions as a key mediator of osteoclast differentiation and activation. By binding to its receptor RANK (Receptor Activator of Nuclear Factor Kappa-B) on osteoclast precursor cells, TNFSF11 stimulates osteoclastogenesis and bone resorption. Additionally, TNFSF11 is involved in the regulation of immune responses, lymph node development, and mammary gland development.

注意事项

  • 破骨分化RANK L建议用量 100 ng/mL; M-CSF(货号：UA040128）建议用量50 ng/mL

  • 生物活性

    • Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The EC50 for this effect is less than 3ng/ml.

    • RANK L诱导 5天 后，进行抗酒石酸酸性磷酸酶（TRAP）染色以特异性鉴定破骨细胞。染色完成后，在光学显微镜下观察，成熟的破骨细胞胞浆呈红色或红紫色。

  • 电泳

    • 2μg (R: reducing condition, N: non-reducing condition).

  • 体积排阻色谱(SEC-HPLC)

    • >95% as determined by SEC-HPLC.

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