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物种
Mouse分子别名
Platelet-derived growth factor subunit B,PDGF subunit B,PDGF-2,Platelet-derived growth factor B chain,Platelet-derived growth factor beta polypeptide,Proto-oncogene c-Sis,Accession
P31240表达序列
Ser82-Thr190表达宿主
Yeast分子量
15-17 kDa (Reducing)
纯度
>95% by SDS-PAGE.内毒素含量
<0.1EU/μg活性
EC50 < 2ng/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
4mM HAc
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
Cell Commun Signal. 2013 Dec 20:11:97.
Platelet-derived growth factor BB (PDGF-BB) is a member of the PDGF family of growth factors that play a crucial role in cell proliferation, migration, and angiogenesis. PDGF-BB is a homodimeric protein that is released by platelets, macrophages, and endothelial cells in response to tissue injury or inflammation. It acts by binding to its receptors, PDGFR-α and PDGFR-β, leading to the activation of downstream signaling pathways involved in cell growth and tissue repair. PDGF-BB is involved in various physiological processes, such as wound healing, tissue regeneration, and vascular development, as well as in pathological conditions, including fibrosis, atherosclerosis, and cancer.
生物活性
Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblast cells. The EC50 for this effect is less than 2ng/ml.
电泳
2μg (R: reducing condition, N: non-reducing condition).
ELISA
Immobilized PDGF-BB Protein, Human (Cat. No. UA040334) at 2.0μg/mL (100μL/well) can bind PDGF R alpha/PDGFRA Fc Chimera Protein, Human (Cat. No. UA010718) with EC50 of 10.5-42.7ng/mL.
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