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物种
Human分子别名
Receptor-type tyrosine-protein kinase FLT3; FL cytokine receptor; Fetal liver kinase-2 (FLK-2); Fms-like tyrosine kinase 3 (FLT-3); Stem cell tyrosine kinase 1 (STK-1);Accession
P36888表达序列
Asn27-Asn541, with C-terminal hIgG1 Fc.
表达宿主
HEK293分子量
100-120 kDa (Reducing)
纯度
>95% by SDS-PAGE and HPLC.内毒素含量
<0.1EU/μg活性
EC50 = 7-12 ng/ml.标记
Unconjugated标签
Human IgG1 Fc性状
Lyophilized Powder缓冲体系
50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose.
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
Immunity. 2002 Oct;17(4):463-72.
Fms-like tyrosine kinase 3 (Flt-3), also known as fetal liver kinase 2 (Flk-2), is a receptor tyrosine kinase that plays a critical role in hematopoiesis and immune cell development. Flt-3 is primarily expressed on hematopoietic stem cells and early progenitor cells, where it regulates cell proliferation, differentiation, and survival. Activation of Flt-3 by its ligand, Flt-3 ligand (Flt-3L), leads to the activation of downstream signaling pathways involved in hematopoietic cell growth and differentiation. Flt-3 is essential for the development of various immune cell lineages, including dendritic cells, natural killer cells, and B cells.
电泳
2μg (R: reducing condition, N: non-reducing condition).
体积排阻色谱(SEC-HPLC)
>95% as determined by SEC-HPLC.
ELISA
Measured by its binding ability in a functional ELISA. When Recombinant Human FLT3L is immobilized 2.5µg/mL (100 µl/well), Recombinant Human FLT3/FLK2 Fc binds with an EC50 of 0.007-0.012 ug/ml.
Immobilized FLT-3L Protein, Mouse (Cat. No. UA040159) at 1.0μg/mL (100μL/well) can bind Flt-3/Flk-2 Protein, Human (UA040316) with EC50 of 2.11-3.21 ng/mL.
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