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物种
Human分子别名
Pro-interleukin-16,Interleukin-16,IL-16,Lymphocyte chemoattractant factor (LCF)Accession
Q14005表达序列
Met1203-Ser1332表达宿主
E.coli分子量
18 kDa (Reducing)
纯度
>95% by SDS-PAGE.内毒素含量
<0.1EU/μg活性
EC50 = 0.06-0.10 μg/ml.标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20mM Tris, 300mM NaCl pH8.0溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
Proc Natl Acad Sci U S A. 1994 May 24;91(11):5109-13.
Interleukin-16 (IL-16) is a cytokine that plays a key role in regulating immune responses and inflammation. It is produced by various immune cells, including CD4+ T cells, mast cells, and eosinophils. IL-16 functions as a chemoattractant for CD4+ T cells, promoting their migration to sites of inflammation. Additionally, IL-16 can modulate the activation and proliferation of CD4+ T cells, as well as other immune cells, through interactions with its receptor, CD4. This cytokine is involved in the pathogenesis of various inflammatory conditions, autoimmune diseases, and cancer.
IL-16 exists in two isoforms: the full-length 130 amino acid (130aa) isoform and a shorter 121 amino acid (121aa) isoform. The 130aa isoform is the canonical form of IL-16 and is known for its chemoattractant and immunomodulatory functions. On the other hand, the 121aa isoform lacks the CD4-binding domain present in the 130aa isoform, resulting in reduced CD4-binding capacity and altered biological activity. The 121aa isoform may exhibit different chemotactic properties and signaling capabilities compared to the full-length 130aa isoform.
电泳
2μg (R: reducing condition, N: non-reducing condition).
ELISA
Measured by its binding ability in a functional ELISA. When Recombinant Human IL-16 is immobilized 5µg/mL (100 µl/well), Recombinant Human CD4/LEU3 His binds with an EC50 of 0.06-0.10μg/ml.
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