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TGF-β3 Protein, Human

ARVD,ARVD1,FLJ16571,LDS5,RNHF,TGFB3,TGFbeta 3,TGF-beta 3,TGF-beta3,TGF-beta-3,transforming growth factor beta-3,transforming growth factor,beta 3.

货号： UA040294

Datasheet COA
  • 价格： ￥1,200
  • 规格：
  • 数量：
斯达特大包装询价 大包装询价

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    ARVD,ARVD1,FLJ16571,LDS5,RNHF,TGFB3,TGFbeta 3,TGF-beta 3, TGF-beta3,TGF-beta-3,transforming growth factor beta-3,transforming growth factor,beta 3.

  • Accession

    P10600

  • 浓度

    0.2mg/ml

  • 表达序列

    Ala301-Ser412

  • 表达宿主

    HEK293

  • 分子量

    12.7 kDa (monomer).

  • 纯度

    ≥95% by SDS-PAGE

  • 活性

    Measured by the(CAGA)12-luciferase reporter assay. The EC50 for this effect is 0.29ng/mL

  • 标记

    Unconjugated

  • 标签

    No Tag

  • 性状

    Liquid

  • 缓冲体系

    Dissolved in sterile 20 mM sodium citrate(pH=2.5) buffer . This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.

  • 储存条件

    Avoid repeated freeze-thaw cycles.
    It is recommended that the protein be aliquoted for optimal storage. 

    12 months from date of receipt, -20 to -70℃ as supplied

  • 稀释度

背景介绍

  • TGF-beta 3 (transforming growth factor-beta 3) is a member of a TGF-beta superfamily subgroup that is defined by their structural and functional similarities (1-5). TGF-beta 3 and its closely related proteins, TGF-beta 1 and --beta 2, act as cellular switches to regulate immune function, cell proliferation, and epithelial--mesenchymal transition (4, 6, 7). The non-redundant biological effects of TGF-beta 3 include involvement in palatogenesis, chondrogenesis, and pulmonary development (1, 2, 7-9). Human TGF--beta 3 cDNA encodes a 412 amino acid (aa) precursor that contains a 20 aa signal peptide and a 392 aa proprotein. The proprotein is processed by a furin-like convertase to generate a 220 aa latency-associated peptide (LAP) and a 112 aa mature TGF--beta 3 (10, 11). Mature human TGF--beta 3 shows 100%, 99%, and 98% aa identity with mouse/dog/horse
    , rat, and pig TGF--beta 3, respectively. TGF-beta 3 is secreted as a complex with LAP. This latent form of TGF-beta 3 becomes active upon cleavage by plasmin, matrix metalloproteases, thrombospondin-1, and a subset of integrins (12). TGF-beta 3 binds with high affinity to TGF-beta RII, a type II serine/threonine kinase receptor. This receptor then phosphorylates and activates type I serine/threonine kinase receptors, TGF--beta RI or ALK--1, to modulate transcription through Smad phosphorylation
    (13-15). The divergent biological effects exerted by individual TGF-beta isoforms is dependent upon the recruitment of co-receptors (TGF--beta RIII and endoglin) and the subsequent initiation of Smad--dependent or -independent signaling pathways (14, 16, 17).

  • 生物活性

    • Measured by (CAGA)12-luciferase reporter assay.

  • 电泳

    • 4 ug/lane protein was resolved
      with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Coomassie blue staining.

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