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物种
Porcine分子别名
Hepatocyte growth factor, Hepatopoietin-A, Scatter factor (SF)Accession
F1SB93表达序列
Gln32-Glu210
表达宿主
E.coli分子量
20.8 kDa (Reducing)
纯度
>95% by SDS-PAGE and HPLC.内毒素含量
<0.1EU/μg活性
EC50 < 40.0ng/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
PBS, 5% trehalose, PH7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
J Cell Biol. 1996 May;133(3):709-18.
Hepatocyte growth factor (HGF) is a potent epithelial mitogen whose actions are mediated through its receptor, the proto-oncogene c-Met. Full-length HGF is a 90-kD protein composed of an NH2-terminal domain, four consecutive kringle domains, and a carboxy-terminal protease-like domain. NK1 was converted to an agonist by adding heparin to the culture medium. The interaction of NK1 and heparin was further studied in BaF3 cells, which express little or no cell surface heparan sulfate proteoglycans. In BaF3 cells transfected with a plasmid encoding human c-Met, heparin and NK1 synergized to stimulate DNA synthesis and cell proliferation.
生物活性
Measured by its ability to induce serum response element (SRE) reporter activity in transfected HEK293T human embryonic kidney cells. The EC50 for this effect is less than 30-37ng/ml.
电泳
2μg (R: reducing condition, N: non-reducing condition).
反相高效液相色谱(RP-HPLC)
>95% as determined by RP-HPLC.
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