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物种
Human分子别名
bFGF,FGF-2,Heparin-binding growth factor 2,Basic fibroblast growth factor,FGFB,HBGF-2,FGF2,Fibroblast Growth Factor 2 (Basic)Accession
P09038表达序列
Ala144-Ser288表达宿主
E.coli分子量
16.3 kDa
纯度
> 95% by SDS-PAGE内毒素含量
<0.1EU/μg活性
EC50 < 0.05ng/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20mM Tris, 500mM NaCl,1mM DTT, pH8.0溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1. J Biol Chem. 1996 Jun 21;271(25):15292-7.
2. Biosci Rep. 2017 Apr 10;37(2): BSR20170173.
3. Biochem Biophys Res Commun. 1986 Mar 13;135(2):541-8.
Basic fibroblast growth factor (bFGF), also known as FGF2, is a member of the fibroblast growth factor (FGF) family. It is a highly specific chemotactic and mitogenic factor for many cell types, appears to be involved in remodeling damaged tissue, such as ulcer healing, vascular repair, traumatic brain injury (TBI). bFGF is a critical component of human embryonic stem cell culture medium. In addition, bFGF protein is a heparin-binding cationic protein involved in a variety of pathological conditions including angiogenesis and solid tumour growth. Thus, bFGF is regarded as a target for cancers chemopreventive and therapeutic strategies.
生物活性
Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblast cells.The EC50 for this effect is less than 0.05ng/ml.
电泳
1μg (R: reducing condition, N: non-reducing condition).
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