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物种
Human分子别名
Lymphotoxin-Alpha,LT-Alpha,TNF-Beta,Tumor Necrosis Factor Ligand Superfamily Member 1,LTA,TNFB,TNFSF1Accession
P01374表达序列
Leu35-Leu205表达宿主
E.coli分子量
18.7 kDa (Reducing)
纯度
>95% by SDS-PAGE and HPLC.内毒素含量
<0.1EU/μg活性
EC50 < 40pg/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20mM Tris, 50mM NaCl, pH8.0溶解方法
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Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
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Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1. Arthritis Res Ther. 2013;15(6): R202.
2. Dev Comp Immunol. 2021 Feb: 115:103884. Epub 2020 Oct 9.
TNF-β, also called lymphotoxin-α (LT-α), is expressed by a variety of cells, including T cells, B cells and natural killer (NK) cells. It can be secreted and, like TNF-α, binds with high affinity to TNF receptors 1 and 2 (TNFR-1 and TNFR-2), and it is transiently expressed on the cell surfaces of activated B and T cells, where it forms a complex with LT-β as an LTα1β2 heterotrimer. TNF-β can activate many kinds of immune cells, and prompt secretion of other cytokines for infection clearance. For some physiological processes, TNF and LTαβ work together as components of an integrated signaling network that is defined in part by communal sharing of receptors and ligands.
生物活性
Measured in a cytotoxicity assay using L929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D，The EC50 for this effect is less than 40pg/ml.
电泳
2μg (R: reducing condition, N: non-reducing condition).
反相高效液相色谱(RP-HPLC)
>95% as determined by RP-HPLC.
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