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物种
Human分子别名
FGF-16; Fibroblast Growth Factor-16Accession
O43320表达序列
Ala2-Arg207
表达宿主
E.coli分子量
25 kDa (Reducing)
纯度
＞95% by SDS-PAGE & HPLC内毒素含量
<0.1EU/μg活性
EC50 < 5ng/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20mM Tris,500mM NaCl, PH8.0
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1. Alina G Sofronescu. Cardiovasc Res. 2010 Jul 1;87(1).
Fibroblast growth factors (Fgfs), proteins of 150~300 amino acids, play diverse roles in development, repair and metabolism. The human/mouse Fgf family comprises twenty-two members. Most Fgfs mediate biological responses by binding to and activating Fgf receptors (Fgfrs) in a paracrine manner. Among paracrine Fgfs, Fgf16 is predominantly expressed in the heart. Fgf16 expression is weakly detected in the embryonic heart and much more abundant at adult stages than embryonic stages. These findings indicate potential roles in the heart.
生物活性
Measured in a cell proliferation assay using Balb/3T3 mouse fibroblast cells, the EC50 for this effect is less than 10 ng/ml.
电泳
反相高效液相色谱(RP-HPLC)
>95% as determined by RP-HPLC.
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