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物种
Rat分子别名
FGF-10,Fibroblast Growth Factor-10,FGFA,Keratinocyte growth factor-2,KGF2Accession
P70492表达序列
Gln37-Ser215
表达宿主
E.coli分子量
23kDa (Reducing)
纯度
＞95% by SDS-PAGE&HPLC内毒素含量
<0.1EU/μg活性
EC50 < 5ng/ml.标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20mM Tris, 200mM NaCl, pH8.0
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1、Hubert F. et al. (2022) FGF10 promotes cardiac repair through a dual cellular mechanism increasing cardiomyocyte renewal and inhibiting fibrosis. Cardiovasc Res. 118(12): 2625-2637.
2、Sun W. et al. (2019) Cytokine fibroblast growth factor 10 (FGF10) polymorphisms are associated with risk of myopia in young children. J Cell Biochem. 120(9): 15241-15247.
Fibroblast growth factor 10 (FGF-10), a secreted heparin-binding protein, is a member of the fibroblast growth factor (FGF) family and is also known as Keratinocyte Growth Factor-2 (KGF-2). FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-10 exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. Studies revealed that genetic variants of FGF10 were associated with extreme myopia in adults.
生物活性
Determined by a cell proliferation assay using HaCat cells. The expected EC50 for this effect is 7-13ng/ml.
电泳
1μg (R: reducing condition, N: non-reducing condition).
反相高效液相色谱(RP-HPLC)
>95% as determined by RP-HPLC.
ELISA
Immobilized FGF-10 Protein, Rat (Cat. No. UA040262) at 5μg/mL (100μL/well) can bind FGF-R2(IIIb) Fc Chimera Protein, Human (Cat. No. UA011251) with EC50 of 14.19-30.85 ng/mL.
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