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TRAIL Protein, Mouse

TNF-related apoptosis-inducing ligand,Protein TRAIL,CD_antigen: CD253,Tnfsf10

价格 ￥720.00 （ 供应商现货 ： 3-5个工作日）
货号 UA040256
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Mouse

  • 分子别名

    TNF-related apoptosis-inducing ligand; Protein TRAIL; CD_antigen: CD253; Tnfsf10

  • Accession

    P50592

  • 表达序列

    Arg119-Asn291

  • 表达宿主

    E.coli

  • 分子量

    19kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE and HPLC

  • 内毒素含量

    <0.1EU/μg

  • 活性

    EC50 < 1ng/ml

  • 标记

    Unconjugated

  • 标签

    No Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    20mM Tris, 300mM NaCl, 1mM DTT, pH8.0

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.

    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.

    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1. Andrew Thorburn (2007) J Thorac Oncol. 2(6):461-5.

背景介绍

  • Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), also known as CD253 or TNFSF10, can be produced and secreted by most normal tissue cells. TRAIL proteins are available in two forms: membrane-bound (full-length TRAIL) and soluble (sTRAIL). In vivo, only the membrane-bound form exists, while the soluble polypeptide (sTRAIL), which is formed in the extracellular region of TRAIL, has the full biological activity of the membrane-bound TRAIL. Both of them are able to bind to specific receptors on the cell membrane and rapidly induce the expression of TRAIL through intracellular signal transduction. They can rapidly induce apoptosis in cells expressing TRAIL-specific receptors. TRAIL has a broad spectrum of action and can induce apoptosis in a variety of tumor cells in the colon, lung, breast, kidney, brain and skin, and is considered as a promising broad-spectrum anti-cancer molecule.

  • 生物活性

    • Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D, The EC50 for this effect is less than 1ng/ml.

  • 电泳

    • 1μg (R: reducing condition, N: non-reducing condition).

  • 体积排阻色谱(SEC-HPLC)

    • >95% as determined by SEC-HPLC.

  • ELISA

    • Immobilized TRAIL Protein, Mouse (Cat. No. UA040256) at 5.0μg/mL (100μL/well) can bind TNFRSF10B/TRAIL R2 Fc Chimera Protein, Mouse (Cat. No. UA010499) with EC50 of 5.20-14.37 ng/mL.

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