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物种
Mouse分子别名
Proheparin-binding EGF-like growth factor (HB-EGF)Accession
Q06186表达序列
Asp63-Leu148
表达宿主
E.coli分子量
14kDa (Reducing)
纯度
＞95% by SDS-PAGE and HPLC内毒素含量
<0.1EU/μg活性
EC50 < 1ng/ml.标记
Unconjugated性状
Lyophilized Powder缓冲体系
20mM Tris, 300mM NaCl, pH8.0, 1mM DTT
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
Cancer Metastasis Rev. 2018 Sep;37(2-3):385-395.
Proheparin-binding EGF-like growth factor (HB-EGF), also known as DTR, DTS and HEGFL, is a 13-15kDa member of the EGF family of mitogens. It is expressed in macrophages, monocytes, endothelial cells and muscle cells. HB-EGF signals through the EGF receptor to stimulate the proliferation of smooth muscle cells, epithelial cells and keratinocytes. Compared to EGF, HB-EGF binds to the EGF receptor with a higher affinity and has been shown to be more mitogenic, likely due to its ability to bind to heparin and heparin sulfate proteoglycans. It has been shown to play a role in wound healing, cardiac hypertrophy and heart development and function. The transmembrane form of HB-EGF is the unique receptor for diptheria toxin and functions in juxtacrine signaling in cells. Both forms of HB-EGF participate in normal physiological processes and in pathological processes including tumor progression and metastasis, organ hyperplasia, and atherosclerotic disease. HB-EGF can bind two locations on cell surfaces, heparan sulfate proteoglycans and EGF-receptor effecting cell to cell interactions.
电泳
2μg (R: reducing condition, N: non-reducing condition).
体积排阻色谱(SEC-HPLC)
>95% as determined by SEC-HPLC.
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