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物种
Human分子别名
Wnt3a Surrogate; Wnt Surrogate-Fc Fusion Protein; Human Wnt3a Surrogate Protein; Wnt Surrogate Fc Chimera,Human表达序列
Lys177-His266
表达宿主
HEK293分子量
57.5kDa (Reducing)
纯度
＞95% by SDS-PAGE.内毒素含量
<0.1EU/μg标记
Unconjugated性状
Lyophilized powder缓冲体系
25mM Tris.pH8.2,500mM NaCl,5%海藻糖
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
Obrecht-Sturm, Schömig, Mynarek et al Neuro Oncol (2024)
Willert, K. and Nusse, R. (2012) Cold Spring Harb. Perspect. Biol. 4:a007864
Chen, Lee, Ouyang et al Antib Ther (2024) 7 (1), 88-95
Wnt surrogate protein is an engineered Fc fusion protein, which activates similar Wnt signaling pathways as most commercially available recombinant Wnt3a proteins or Wnt-conditioned media, while requiring a fraction of the amount of protein or hassle. It is necessary for cell proliferation and differentiation and the self-renewal of stem cells and is widely used for supporting organoid culture, including intestinal, stomach, liver, lung organoids. Modulation of WNT signaling pathways has potential for treatment of degenerative diseases and tissue injuries. This engineered Wnt surrogate protein can be selective for individual FZD or a subfamily of FZDs in combination with LRP5 or LRP6, depending on the binder specificities, and may be used in any of a variety of therapeutic methods for the treatment of diseases.
Recombinant Human Wnt3a Surrogate Protein activates Wnt signaling pathways and is necessary for cell proliferation and differentiation and the self-renewal of stem cells. It is widely used for supporting organoid culture, including intestinal, stomach, liver, lung organoids.
生物活性
Measured by its ability to induce TCF reporter activity in HEK293T human embryonic kidney cells. The EC50 for this effect is less than 90ng/ml.
电泳(SDS-PAGE)
1μg (R: reducing condition, N: non-reducing condition).
体积排阻色谱(SEC-HPLC)
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