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物种
Mouse分子别名
fgf17b; zgc:101759Accession
P63075表达序列
Thr23-Thr216
表达宿主
E.coli分子量
25kDa (Reducing)
纯度
＞95% by SDS-PAGE and HPLC.内毒素含量
<0.1EU/μg活性
EC50 < 10ng/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20mM Tris, 500mM NaCl, pH8.0
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1. K Scearce-Levie and E D Roberson (2008) Genes Brain Behav. 7(3):344-54.
2. Nobuyuki Itoh and David M Ornitz (2008) Dev Dyn. 237(1):18-27.
Fibroblast Growth Factors (FGFs) are polypeptides with diverse activities in development and physiology. The mammalian Fgf family can be divided into the intracellular Fgf11/12/13/14 subfamily (iFGFs), the hormone-like Fgf15/21/23 subfamily (hFGFs), and the canonical Fgf subfamilies, including Fgf1/2/5, Fgf3/4/6, Fgf7/10/22, Fgf8/17/18, and Fgf9/16/20. Recent evidence suggests that Fgf17 is important in neural patterning. During embryonic development, Fgf17 is expressed in the patterning centers for the rostral forebrain and the midbrain/hindbrain. Studies of Fgf17-deficient mice showed that Fgf17 is required for development of the cerebellar vermis and inferior colliculus and for the regionalization of frontal cortex. Fgf17 ablation reduced the size of the dorsal frontal cortex and the extent of frontal cortex projections to subcortical targets.
生物活性
Measured in a cell proliferation assay using NIH-3T3 mouse fibroblast cells. The EC50 for this effect is 144-217 ng/mL.
Measured in a cell proliferation assay using Balb/3T3 mouse fibroblast cells, the EC50 for this effect is 114-279ng/ml.
电泳
2μg (R: reducing condition, N: non-reducing condition).
反相高效液相色谱(RP-HPLC)
>95% as determined by RP-HPLC.
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