分享产品介绍 评论(0)
物种
Mouse分子别名
FGF18; FGF-18; fibroblast growth factor 18; zFGF5Accession
O89101表达序列
Glu28-Gly207
表达宿主
E.coli分子量
21 kDa (Reducing)
纯度
＞95% by SDS-PAGE and HPLC.内毒素含量
<0.1EU/μg活性
EC50 < 6ng/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
1×PBS, pH7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
Davidson D. et al. (2005) Fibroblast growth factor (FGF) 18 signals through FGF receptor 3 to promote chondrogenesis. J Biol Chem. 280(21): 20509-15.
Liu Z. et al. (2007) FGF18 is required for early chondrocyte proliferation, hypertrophy and vascular invasion of the growth plate. Dev Biol. 302(1): 80-91.
Fibroblast Growth Factor 18 (FGF-18) plays an important role in skeletal development and bone homeostasis. Mature human FGF-18 shares 99% amino acid sequence identity with mouse and rat FGF-18. It is expressed in embryonic somites and the neural fold, adult lung, cerebellar and hippocampal neurons, hair follicle root sheath cells, and osteogenic mesenchymal cells. FGF-18 is a heparin-binding growth factor that belongs to the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-18 is required for normal skeletal development. Recombinant Human FGF-18 derived from E.coli is a 20 kDa protein consisting of 173 amino acid residues, resulting from C-terminal truncation of the full length protein.
生物活性
Measured in a cell proliferation assay using Balb/3T3 mouse fibroblast cells, the EC50 for this effect is 106-209ng/ml.
电泳
1μg (R: reducing condition, N: non-reducing condition).
反相高效液相色谱(RP-HPLC)
>95% as determined by RP-HPLC.
评论(0)