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IL-10 Protein, Porcine

Interleukin-10,Cytokine synthesis inhibitory factor(CSIF)

价格 ￥890.00 （ 供应商现货 ： 3-5个工作日）
货号 UA040242
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Porcine

  • 分子别名

    Interleukin-10, Cytokine synthesis inhibitory factor(CSIF)

  • Accession

    Q29055

  • 表达序列

    Ser19-Asn175

  • 表达宿主

    E.coli

  • 分子量

    18 kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE

  • 内毒素含量

    <0.1EU/μg

  • 活性

    EC50 < 2ng/ml.

  • 标记

    Unconjugated

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, PH7.4

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.

    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.

    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1. Kim JM, Brannan CI, Copeland NG, et al. 1992. J Immunol. 148:3618-23.
    2. Tan JC, Braun S, Rong H, et al. 1995. J Biol Chem. 270:12906-11.
    3. Pestka S, Krause CD, Sarkar D, et al. 2004. Annu Rev Immunol. 22:929-79.

背景介绍

  • Interleukin 10(IL10), also known as cytokine synthesis inhibitory factor (CSIF), is a secreted protein and belongs to the IL-10 family. IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. IL-10 is an anti-inflammatory TH2 cytokine that has a critical role in limiting the immune response to pathogens to prevent host damage. As IL-10 in produced in several T helper populations, it is proposed that it provides a feedback loop to limit the effector functions of macrophages and DCs on T cells. Once expressed, IL-10 signals through the IL-10 receptor (IL-10R) to activate STAT3. As IL-10 is a strong inhibitor of inflammation, it has become a viable biomarker for various diseases and conditions as well as a therapeutic molecule for certain conditions. In addition to elevated levels in parasitic infection, high expression levels of IL-10 are also found in retroviral infections inducing immunodeficiency. The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations.

  • 生物活性

    • Measured in a cell proliferation assay using MC/9 mouse mast cells. The ED50 for this effect is 8‑12 ng/mL.

  • 电泳

    • 2μg (R: reducing condition, N: non-reducing condition).

  • ELISA

    • Immobilized IL-10 Protein, Porcine (Cat. No. UA040242) at 5.0μg/mL (100μL/well) can bind IL10RA/CD210 His Tag Protein, Human (Cat. No. UA010657) with EC50 of 10.38-16.16 ng/mL.

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