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物种
Human分子别名
IL-7,Interleukin-7Accession
P13232表达序列
Asp26-His177表达宿主
E.coli分子量
18kDa (Reducing)
纯度
＞95% by SDS-PAGE&RP-HPLC内毒素含量
<0.1EU/μg活性
<0.5ng/ml标记
Unconjugated标签
No Tag性状
Lyophilized powder缓冲体系
PBS, pH7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1.Tan J, Wang C, Jin Y, Xia Y, Gong B, Zhao Q. Optimal combination of MYCN differential gene and cellular senescence gene predicts adverse outcomes in patients with neuroblastoma. Front Immunol. 2023 Nov 16; 14:1309138.
2.Padovano C, Bianco SD, Sansico F, De Santis E, Tamiro F, Colucci M, Totti B, Di Iasio S, Bruno G, Panelli P, Miscio G, Mazza T, Giambra V. The Notch1 signaling pathway directly modulates the human RANKL-induced osteoclastogenesis. Sci Rep. 2023 Dec 1;13(1):21199.
IL7, also known as interleukin 7, is a hematopoietic growth factor that belongs to the IL-7/IL-9 family. It is secreted by stromal cells in the bone marrow and thymus. It is also produced by keratinocytes, dendritic cells, hepatocytes, neurons, and epithelial cells, but is not produced by lymphocytes. Human IL‑7 cDNA encodes 177 amino acids (aa) that include a 25 aa signal peptide. Human IL-7 shares approximately 60-63% aa sequence identity with mouse, rat, canine and feline IL-7, and 72-76% with equine, bovine, ovine, and porcine IL-7. IL7 stimulates the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. Like other common gamma-chain cytokines like IL-2 and IL-15, IL-7 and its receptor, IL-7R, have been used in a variety of immunotherapy applications, often in fluid tumors and in some instances of solid tumor models.
生物活性
Measured in a cell proliferation assay using Murine 2E8 cells,The EC50 for this effect is less than 0.5ng/ml.
电泳
1μg (R: reducing condition, N: non-reducing condition).
反相高效液相色谱(RP-HPLC)
SPR
Protein A Chip captured IL-7R alpha/CD127 Fc Chimera Protein, Human (Cat. No. UA010892), can bind IL-7 Protein, Human (Cat. No. UA040234) with an affinity constant of 40.35nM as determined in SPR assay.
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