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产品中心 细胞因子 生长因子

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FGF-21 Protein, Rat

Fibroblast growth factor 21

价格 ￥1,200.00 （ 供应商现货 ： 3-5个工作日）
货号 UA040231
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Rat

  • 分子别名

    Fibroblast growth factor 21

  • Accession

    Q8VI80

  • 表达序列

    Ala29-Ser208

  • 表达宿主

    E.coli

  • 分子量

    20kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE&RP-HPLC

  • 内毒素含量

    <0.1EU/μg

  • 活性

    EC50 < 0.2μg/ml

  • 标记

    Unconjugated

  • 标签

    No Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    20mM PB,500mM NaCl,pH 7.4.

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.

    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.

    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1. J Clin Invest. 2005 Jun;115(6):1627-35. . Epub 2005 May 2.
    2. Exp Gerontol. 2020 Oct 1;139:111022.

背景介绍

  • Fibroblast Growth Factor 21(FGF-21) is a member of the fibroblast growth factor (FGF) family and it is involved in the modulation of cell proliferation, growth, and differentiation as well as cell metabolism. This protein is a secreted endocrine factor that functions as a major metabolic regulator, and stimulates the uptake of glucose in adipose tissue. FGF21 binds to FGFR1c complexed with β-Klotho expressed in adipocytes to activate the canonical FGF signaling pathway (ERK1/2) and the AMPK/PGC1α pathway. Therefore, FGF-21 is being pursued as a therapeutic target for diabetes and obesity.

  • 生物活性

    • Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast cells. The EC50 this effect is less than 0.2μg/ml in the presence of 2μg/ml recombinant Klotho β and 20μg/ml heparin.

  • 电泳

    • 2μg (R: reducing condition, N: non-reducing condition).

  • 反相高效液相色谱(RP-HPLC)

    • 96.4%

  • SPR

    • Anti-His antibody Immobilized on CM5 Chip captured Klotho β Protein, Mouse (Cat. No. UA040364), can bind FGF-21 Protein, Rat (Cat. No. UA040231) with an affinity constant of 38.48nM as determined in SPR assay.

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