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物种
Human分子别名
R-spondin-2; RSPO2; Roof plate-specific spondin-2; hRspo2; PRO34209Accession
Q6UXX9表达序列
Gln22-Gly205, with a Flag tag at the C-terminus
表达宿主
CHO分子量
22.3 KDa
纯度
＞95% by SDS-PAGE
性状
Lyophilized Powder缓冲体系
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
溶解方法
Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
储存条件
- Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
- Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
- Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
R-spondin-2, also known as RSPO2, synergizes with Wnt to activate beta-catenin. R-spondins are a family of four matricellular proteins produced by a variety of cell-types. The four R spondin secreted ligands (RSPO1–RSPO4) act via their cognate LGR4, LGR5 and LGR6 receptors to amplify WNT signalling. Rspo2 in FGF pathway regulation in vivo Overexpressed Rspo2 inhibited elongation of Xenopus ectoderm explants and Erk1 activation in response to FGF. R-spondin2 (RSPO2) plays a crucial role in the activation of the WNT/β-catenin pathway and oncogenesis, RSPO2 in GC specimens and cell lines is closely related to tumor invasion and migration and that RSPO2 promotes EMT in gastric cancer cells by activating WNT/β-catenin signaling.
生物活性
Measured by its ability to induce Topflash reporter activity in 293 human embryonic kidney cells. The typical ED50 is 86 ng/mL in the presence of 3.24 ng/mL Recombinant Human Wnt3a V3
电泳
Greater than 95% as determined by SEC-HPLC.
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