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物种
Rat分子别名
IGIF, IL-18, IL1F4Accession
P97636-1表达序列
His37-Ser194
表达宿主
E.coli分子量
18kDa (Reducing)
纯度
＞95% by SDS-PAGE内毒素含量
<0.1EU/μg活性
<40ng/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20mM Tris, 200mM NaCl, pH8.0
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1.Acta Biochim Pol 2016;63(1):59-63.
2.Epub 2016 Feb 17. 2. Int J Mol Sci 2019 Feb 2;20(3):649.
Interleukin 18 (IL-18) is a pleiotropic cytokine involved in the regulation of innate and acquired immune response. In the milieu of IL-12 or IL-15, IL-18 is a potent inducer of IFN-gamma in natural killer (NK) cells and CD4 T helper (Th) 1 lymphocytes. However, IL-18 also modulates Th2 and Th17 cell responses, as well as the activity of CD8 cytotoxic cells and neutrophils, in a host microenvironment-dependent manner. IL-18 is a cytokine that stimulates various cell types and has pleiotropic functions. Recently, IL-18 has also been shown to execute specific effects in pancreatic diseases, including acute and chronic pancreatitis, as well as pancreatic cancer.
生物活性
Measured by its ability to induce IFN-γ secretion by KG-1human myelomonocyte cells.The EC50 for this effect is less than 100ng/ml.
电泳
1μg (R: reducing condition, N: non-reducing condition).
ELISA
Immobilized IL-18 Protein, Rat (Cat. No. UA040226) at 2.0μg/mL (100μL/well) can bind IL-18BP Fc Chimera Protein, Human (Cat. No. UA010587) with EC50 of 6.32-22.69 ng/mL.
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