IL-12 Protein,Mouse
IL-12a Protein,Mouse,IL-12b Protein,IL-12p35 Protein,IL-12p40 Protein,IL12,IL-12,subunit p35,IL12A,interleukin 12,p35,CLMF p35,CLMF1,Cytotoxic lymphocyte maturation factor 35 kDa subunit,IL-12 subunit p35,NKSF1,NK cell stimulatory factor chain 1,interleukin-12 alpha chain,interleukin-12 subunit alpha,natural killer cell stimulatory factor 1,35 kD subunit,p35 Protein,p40 Protein
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物种
Mouse分子别名
IL-12a Protein, Mouse; IL-12b Protein, Mouse; IL-12p35 Protein, Mouse; IL-12p40 Protein, Mouse; IL12; IL-12; IL-12, subunit p35; IL12A; interleukin 12, p35; CLMF p35; p35; CLMF1; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12 subunit p35; NKSF1; NK cell stimulatory factor chain 1; interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; p35 Protein, Mouse; p40 Protein, MouseAccession
P43432 , P43431表达序列
p40: Met23-Ser335; p35: Arg23-Ala215, with C-terminal His Tag
表达宿主
CHO分子量
P40: 35.8 kDa (Reducing)
P35: 22.5 kDa (Reducing)
纯度
＞95% by SDS-PAGE and HPLC内毒素含量
<0.1EU/μg活性
EC50 < 0.6ng/ml.标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4.
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1. Airoldi, I. et al. (2000) Journal of Immunology 165:6880.
2. Wolf, S.F. et al. (1991) Journal of Immunology 146:3074.
3. Gearing, D. et al. (1991) Cell 66:9.
4. Merberg, D. et al. (1992) Immunology Today 13:78.
5. Gubler, U. et al. (1991) Proc. Natl. Acad. Sci. 88:4143.
IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) from T cells and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-γ. T cells that produce IL-12 have a coreceptor, CD30, which is associated with IL-12 activity. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 mediates enhancement of the cytotoxic activity of NK cells and CD8+ cytotoxic T lymphocytes.
生物活性
Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 11-20 ng/mL.
电泳
2μg (R: reducing condition, N: non-reducing condition).
反相高效液相色谱(RP-HPLC)
>95% as determined by RP-HPLC.
ELISA
Immobilized IL-12 Protein, Mouse (Cat. No. UA040218) at 1.0μg/mL (100μL/well) can bind IL-12 R beta 1 Fc Chimera Protein, Human (Cat. No. UA010956) with EC50 of 0.05-0.15μg/mL.
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