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产品中心 细胞因子 生长因子

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NT-3 Protein, Human

HDNF,Nerve growth factor 2,NGF-2,Neurotrophic factor,NTF3

价格 ￥1,180.00 （ 供应商现货 ： 3-5个工作日）
货号 UA040216
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    HDNF,Nerve growth factor 2,NGF-2,Neurotrophic factor,NTF3

  • Accession

    P20783

  • 表达序列

    Tyr139-Thr257 YAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEARPVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT

  • 表达宿主

    E.coli

  • 分子量

    15kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE

  • 内毒素含量

    <0.1EU/μg

  • 活性

    0.015-0.02μg/ml

  • 标记

    Unconjugated

  • 标签

    No Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    20mM Tris, 500mM NaCl, pH8.0

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    ·12 months from date of receipt, -20 to -70 °C as supplied.

    ·1 month, 2 to 8 °C under sterile conditions after reconstitution.

    ·Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1. Elizabeth Hernández-Echeagaray (2020) Vitam Hor. 114:71-89.

    2. F Marmigère. (2001) Neuroendocrinology 74(1):43-54.

背景介绍

  • Neurotrophin-3 (NT-3) belongs to a family of growth factors called neurotrophins whose actions are centered in the nervous system. The neurotrophin family includes nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), neurotrophin-4/5 (NT-4/5), neurotrophin-6(NT-6) and the recently cloned neurotrophin-7 Most of biological effects of neurotrophins are mediated by high affinity tyrosine kinase (Trk) receptors, although some of them require the binding to a low affini tyreceptor (p75LNTR). NGF binds to TrkA receptors, BDNF preferentially activates TrkB receptors, while NT-3 mainly interacts with TrkC receptors, and at lower affinity with TrkB receptors. NT-3 is structurally related to other neurotrophins like brain-derived neurotrophic factor. The expression of NT-3 starts with the onset of neurogenesis and continues throughout life. A wealth of information links NT-3 to the growth, differentiation, and survival of hippocampal cells as well as sympathetic and sensory neurons.

  • 电泳

    • 1μg (R: reducing condition, N: non-reducing condition).

  • ELISA

    • Measured by its binding ability in a functional ELISA. When Recombinant Human NT-3 is immobilized 1µg/mL (100 µl/well), Recombinant Human TrκB Fc, Human binds with an EC50 of 0.015-0.02μg/ml.

    • Immobilized NT-3 Protein, Human(Cat. No. UA040216) at 1.0μg/mL (100μL/well) can bind TrkC Fc Chimera Protein, Human (UA010243) with EC50 of 7.48-18.2 ng/mL.

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