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物种
Human分子别名
SGRF,IL-23p19,CLMF p40,IL-12 subunit p40,NKSF2Accession
P29460, Q9NPF7表达序列
Ile23-Ser328&Arg20-Pro189, with the C-terminus His Tag表达宿主
HEK293分子量
60-90 KDa, reducing conditions
纯度
＞95% by SDS-PAGE
内毒素含量
<0.1EU/μg活性
The ED50 for this effect is 300-900 ng/ml标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
20 mM Tris-HCl, 15% Trehalose, 0.05% Tween80, pH8.5溶解方法
- Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
- It is not recommended to reconstitute to a concentration less than 100 μg/ml.
- Dissolve the lyophilized protein in distilled water.
- Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
储存条件
- Lyophilized protein should be stored at ≤ -20℃, stable for one year after receipt.
- Reconstituted protein solution can be stored at 2-8℃ for 2-7 days.
- Aliquots of reconstituted samples are stable at ≤ -20℃ for 3 months.
Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naive and memory human T cells, the effects of IL-23 is restricted to memory T cells.
生物活性
Measured by its ability to induce STAT reporter activity in 293F human embryonic kidney cells. The ED50 for this effect is 300-900 ng/ml.
引用文献(1)
- Loss of Roquin-1 enhances CXCR5+ PD-1+ Tfh cell differentiation by interfere AMPK function to enhance glycolysis in Neuromyelitis Optica
Hui Li; Peidong Liu; Ruoyu Li; Huiru Hu; Ao Shen; et al
Research Square (Research Square). 2024 Nov 18 .
货号：UA040207产品名称：IL-23 Protein, Human
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