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IL-12 Protein, Human STAR

Interleukin-12 subunit alpha,IL-12A,Cytotoxic lymphocyte maturation factor 35 kDa subunit,CLMF p35,IL-12 subunit p35,NK cell,IL12A,NKSF1 stimulatory factor chain 1

价格 ￥1,350.00 （ 供应商现货 ： 3-5个工作日）
货号 UA040200
规格
数量
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产品介绍

产品介绍 引用文献(1) 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    Interleukin-12 subunit alpha; IL-12A; Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; NK cell; IL12A ; NKSF1 stimulatory factor chain 1

  • Accession

    P29459, P29460

  • 表达序列

    p40:Ile23-Ser328 p35:Arg23-Ser219

  • 表达宿主

    CHO

  • 分子量

    P40:34.7kDa (Reducing) ;P35:22.5kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE

  • 内毒素含量

    <1EU/μg

  • 活性

    EC50 < 0.2 ng/ml

  • 标记

    Unconjugated

  • 标签

    No Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, pH7.4.

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.

    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.

    · Please avoid repeated freeze-thaw cycles.

  • 文献引用

    1. Airoldi, I. et al. (2000) Journal of Immunology 165:6880.

    2. Wolf, S.F. et al. (1991) Journal of Immunology 146:3074.

    3. Gearing, D. et al. (1991) Cell 66:9.

    4. Merberg, D. et al. (1992) Immunology Today 13:78.

    5. Gubler, U. et al. (1991) Proc. Natl. Acad. Sci. 88:4143.

背景介绍

  • IL-12 is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit.IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) from T cells and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-γ. T cells that produce IL-12 have a coreceptor, CD30, which is associated with IL-12 activity.IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 mediates enhancement of the cytotoxic activity of NK cells and CD8+ cytotoxic T lymphocytes. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

  • 生物活性

    • Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The EC50 for this effect is less than 0.2ng/ml.

    • Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The EC50 for this effect is less than 0.2ng/ml.

  • 电泳

    • 2μg (R: reducing condition, N: non-reducing condition).

  • 反相高效液相色谱(RP-HPLC)

    • >95% as determined by HPLC.

  • ELISA

    • Immobilized IL-12, Human(Cat. No. UA040200) at 2.0μg/mL (100μL/well) can bind IL-12 R beta 1 Fc Chimera, Human(Cat. No. UA010956) with EC50 of 0.18-0.23 μg/mL.

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