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物种
Bovine分子别名
TNF-alpha,Tumor necrosis factor ligand superfamily member 2,TNF-a,Cachectin,DIF,TNFA,TNFSF2Accession
Q06599表达序列
Leu78-Leu234表达宿主
E.coli分子量
17 kDa (Reducing)纯度
＞95% by SDS-PAGE内毒素含量
<0.1EU/μg活性
<1ng/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4.溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1.Benyo DF. et al. (1992). Endocrinology. 130(2):854-60.
2.El-Tahan RR, et al. (2016). Springerplus.5(1):1508.
3.Jang DI, et al. (2021). Int J Mol Sci. 22(5):2719.
4.Berguetti T, et al. (2019). Cells. 8(5):500.
TNF alpha is produced by various types of cells including macrophages, monocytes, neutrophils, T cells, and NK-cells. TNF-alpha is critical for normal immune response, abnormal secretion TNF alpha activates synovial fibroblasts, keratinocytes, osteoclasts, induces rheumatoid arthritis, inflammatory bowel disease, psoriatic arthritis (PsA), and noninfectious uveitis (NIU). TNF alpha positively regulates endogenous TNF-α expression levels independently of Pgp efflux activity, induces IHF cells proliferation. TNF alpha in tissues may promote cancer growth, invasion, and metastasis. Besides, TNF alpha stimulates NF-κB pathway via TNFR2 and anti-TNF-α MAb significantly suppresses the tumor development in colitis-associated cancer (CAC) mouse. TNF alpha as a proneurogenic factor activates the SAPK/JNK pathway and can facilitate neuronal replacement and brain repair in response to brain injury.
生物活性
Measured in a cell proliferation assay using L-929 mouse fibrosarcoma cells, the EC50 for this effect is less than 1ng/ml.
电泳
1μg (R: reducing condition, N: non-reducing condition).
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