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物种
Rat分子别名
Ciliary neurotrophic factorAccession
P20294表达序列
Ala2-Met200表达宿主
E.coli分子量
22kDa (Reducing)
纯度
＞95% by SDS-PAGE&RP HPLC内毒素含量
<0.1EU/μg活性
<20ng/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20mM PB, 2mM TCEP, pH 7.5
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1.Prog Retin Eye Res. 2012 Mar;31(2):136-51. Epub 2011 Dec 10.
Ciliary neurotrophic factor (CNTF) is a pluripotent neurotrophic factor originally isolated from chick embryo ciliary neurons. CNTF has potent effects on the development and maintenance of the nervous system, inducing neuronal survival and differentiation by stimulating gene expression of sensory, sympathetic and motor neurons. Ciliary neurotrophic factor (CNTF) is the most extensively studied member of the cytokine family that signal through intracellular chains of the gp130/LIFRβ receptor. A large body of evidence demonstrates that CNTF promotes rod photoreceptor survival in almost all animal models. Recent studies indicate that CNTF also promotes cone photoreceptor survival and cone outer segment regeneration in the degenerating retina and improves cone function in dogs with congenital achromotopsia.
生物活性
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The EC50 for this effect is less than 30ng/ml.
电泳
1μg (R: reducing condition, N: non-reducing condition).
反相高效液相色谱(RP-HPLC)
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