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物种
Human分子别名
Dickkopf-1, Dkk-1, hDkk-1, DKK1Accession
O94907表达序列
Thr32-His266, with N-terminal His Tag
表达宿主
HEK293分子量
37-46kDa (Reducing)
纯度
＞95% by SDS-PAGE内毒素含量
<0.1EU/μg活性
＜60ng/ml标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
PBS, pH7.4.
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1. Fedi P, Bafico A, Soria AN, Burgess WH, Miki T, Bottaro DP, et al. Isolation and Biochemical Characterization of the Human Dkk-1 Homologue, a Novel Inhibitor of Mammalian Wnt Signaling. J Biol Chem (1999) 274(27):19465–72.
2. Hameed A, Brady JJ, Dowling P, Clynes M, O’Gorman P. Bone Disease in Multiple Myeloma: Pathophysiology and Management. Cancer Growth Metastasis (2014) 7:33–42.
3.Pinzone JJ, Hall BM, Thudi NK, Vonau M, Qiang YW, Rosol TJ, et al. The Role of Dickkopf-1 in Bone Development, Homeostasis, and Disease. Blood (2009) 113(3):517–25.
DKK-1 is a 266-amino acid protein with an approximate molecular mass of 26-kDa, and it possesses six secondary structures, which are two alpha helices and four β-sheets. It has the colipase-like fold in C-terminal Cys domain, which likely allows it to serve as an interactive surface. DKK-1 expression appears to be preferential in some adult tissues including bone, placenta, prostate, spleen, and colon. DKK-1 has been shown to play an important role as a Wnt signaling pathway antagonist in several studies. DKK-1 is down-regulated in human colon tumours; hence, it may act as a tumour suppressor. High DKK-1 expression indicated favourable responses to chemotherapy in brain tumours whereas; over-expression of DKK-1 was found in human hepatoblastomas, Wilms’ tumours and multiple myelomas. Moreover, since overexpression of DKK-1 in osteoblasts causes osteopenia, it is also regarded as a negative regulator of normal bone homeostasis.
生物活性
Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293T human embryonic kidney cells. Recombinant Human DKK-1 His Tag inhibits a constant dose of 50 ng/ml of Recombinant Human Wnt‑Surrogate. The EC50 for this effect is less than 60 ng/ml.
电泳
1μg (R: reducing condition, N: non-reducing condition).
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