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物种
Mouse分子别名
Interleukin-33蛋白；IL-33蛋白;Interleukin 33; IL-33; IL33; C9orf26; NKHEV; Interleukin-1 family member 11; DVS27; NF-HEV and IL- 1F11Accession
Q8BVZ5表达序列
Ser109-Ile266
表达宿主
E.coli分子量
18kDa (Reducing)
纯度
＞95% by SDS-PAGE内毒素含量
<0.1EU/μg活性
EC50 < 0.3ng/mL标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20mM PB, 300mM NaCl, pH6.5
溶解方法
-
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
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Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1. Immunol Rev. 2018 Jan;281(1):154-168.
2. Cytokine. 2022 Sep:157:155961. Epub 2022 Jul 14.
Interleukin-33 (IL-33) is a tissue-derived nuclear cytokine from the IL-1 family abundantly expressed in endothelial cells, epithelial cells and fibroblast-like cells, both during homeostasis and inflammation. It functions as an alarm signal (alarmin) released upon cell injury or tissue damage to alert immune cells expressing the ST2 receptor (IL-1RL1). The major targets of IL-33 in vivo are tissue-resident immune cells such as mast cells, group 2 innate lymphoid cells (ILC2s) and regulatory T cells (Tregs). Other cellular targets include T helper 2 (Th2) cells, eosinophils, basophils, dendritic cells, Th1 cells, CD8+ T cells, NK cells, iNKT cells, B cells, neutrophils and macrophages. IL-33 exhibits pleiotropic function in inflammatory diseases and particularly in cancer. IL-33 may play a dual role as both a pro-tumorigenic and anti-tumorigenic cytokine, dependent on tumor and cellular context, expression levels, bioactivity and the nature of the inflammatory environment
生物活性
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The EC50 for this effect is 0.11-0.22 ng/ml
电泳
2μg (R: reducing condition, N: non-reducing condition).
ELISA
Immobilized IL-33 Protein, Mouse (Cat. No. UA040179) at 2.0μg/mL (100μL/well) can bind IL1RL1/ST2 Fc Chimera Protein, Human (Cat. No. UA010464) with EC50 of 33.07-75.85ng/mL.
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