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物种
Human分子别名
IInterleukin-10,IL-10,Cytokine synthesis inhibitory factor,CSIF,IL10,RP11-262N9.1,IL10A,MGC126450,MGC126451,TGIFAccession
P22301表达序列
Ser19-Asn178表达宿主
E.coli分子量
19kDa (Reducing)
纯度
＞95% by SDS-PAGE内毒素含量
<0.1EU/μg活性
EC50 < 2ng/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
PBS, pH6.5
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1. Kim JM, Brannan CI, Copeland NG, et al. 1992. J Immunol. 148:3618-23.
2. Tan JC, Braun S, Rong H, et al. 1995. J Biol Chem. 270:12906-11.
3. Pestka S, Krause CD, Sarkar D, et al. 2004. Annu Rev Immunol. 22:929-79.
Interleukin 10(IL10), also known as cytokine synthesis inhibitory factor (CSIF),is a secreted protein and belongs to the IL-10 family. IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts . IL-10 is an anti-inflammatory TH2 cytokine that has a critical role in limiting the immune response to pathogens to prevent host damage. As IL-10 in produced in several T helper populations, it is proposed that it provides a feedback loop to limit the effector functions of macrophages and DCs on T cells. Once expressed, IL-10 signals through the IL-10 receptor (IL-10R) to activate STAT3. As IL-10 is a strong inhibitor of inflammation, it has become a viable biomarker for various diseases and conditions as well as a therapeutic molecule for certain conditions. In addition to elevated levels in parasitic infection, high expression levels of IL-10 are also found in retroviral infections inducing immunodeficiency. The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations.
生物活性
Measured in a cell proliferation assay using murine MC/9 cells. The EC50 this effect is less than 2ng/ml.
电泳
1μg (R: reducing condition, N: non-reducing condition).
ELISA
Immobilized IL-10, Human (Cat. No. UA040176) at 5.0μg/mL (100μL/well) can bind IL10RA/CD210 His Tag, Human (Cat. No. UA010657) with EC50 of 13.88-19.80 ng/mL.
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