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物种
Mouse分子别名
NFα蛋白,Tumor Necrosis Factor,Cachectin,TNF-Alpha,Tumor Necrosis Factor Ligand Superfamily Member 2,TNF-a,Tumor Necrosis Factor,Membrane Form,Tumor Necrosis Factor,Soluble Form,Tnf,Tnfa,Tnfsf2Accession
P06804表达序列
Asp89-Leu235表达宿主
E.coli分子量
17kDa (Reducing)
纯度
＞95% by SDS-PAGE and SEC-HPLC内毒素含量
<0.1EU/μg活性
EC50＜15pg/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20mM PB, 300mM NaCl, 2mM TCEP, pH7.5
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1.Microsc Res Tech. 2000 Aug 1;50(3):184-95.
2.Methods Mol Biol. 2021;2248:271-279.
Tumor Necrosis Factor (TNF) is a member of the Tumor Necrosis Factor family. TNF exists as a homotrimer and interacts with SPPL2B. TNF is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. TNF is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes throught its effects on insulin resistance and fatty acid metabolism.
生物活性
Measured in a cell proliferation assay using L-929 mouse fibrosarcoma cells, the EC50 for this effect is less than 10pg/ml.
电泳
2μg (R: reducing condition, N: non-reducing condition).
体积排阻色谱(SEC-HPLC)
>95% as determined by SEC-HPLC.
引用文献(1)
- Novel bispecific nanobody mitigates experimental intestinal inflammation in mice by targeting TNF-α and IL-23p19 bioactivities
Wang J, Kang G, Lu H, de Marco A, Yuan H, Feng Z, Gao M, Wang X, Wang H, Zhang X, Wang Y, Zhang M, Wang P, Feng Y, Liu Z, Cao X, Huang H.
Clin Transl Med. 2024 Mar 01 .
影响因子： 6.8
货号：UA040173产品名称：TNF-α Protein, Mouse
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