IL-2 Protein, Mouse

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货号： UA040171

Datasheet COA

大包装询价

产品介绍引用文献(1) 评论(0)

产品规格

物种

Mouse
分子别名

Interleukin-2蛋白；IL-2蛋白;aldesleukin; interleukin 2; interleukin-2; IL-2; IL2; T-cell growth facter; T cell growth factor; TCGF ; REF: C1025
Accession

P04351
表达序列

Ala21-Gln169
表达宿主

E.coli
分子量

18 kDa (Reducing)
纯度

＞95% by SDS-PAGE
内毒素含量

<0.1EU/μg
标记

Unconjugated
标签

No Tag
性状

Lyophilized Powder
缓冲体系

20mM Sodium Citrate, pH3.0.
溶解方法

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用

1. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
2. Gaffen, S.L. and K.D. Liu (2004) Cytokine 28:109.
3. Kashima, N. et al. (1985) Nature 313:402.
4. Matesanz, F. et al. (1993) Immunogenetics 38:300.
5. Mosmann, T.R. et al. (1987) J. Immunol. 138:1813.
6. Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
7. Wang, X. et al. (2005) Science 310:1159.
8. Bodnar, A. et al. (2008) Immunol. Lett. 116:117.
9. Jaleco, S. et al. (2003) J. Immunol. 171:61.
10. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
11. Laurence, A. et al. (2007) Immunity 26:371.
12. Kryczek, I. et al. (2007) J. Immunol. 178:6730.
13. Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
14. Fehervari, Z. et al. (2006) Trends Immunol. 27:109.

背景介绍

Interleukin 2 (IL 2), also termed T-cell growth factor, is a member of the cytokine family which includes IL-4, IL-7, IL-9, IL-15 and IL-21. Each member of this family has a four alpha helix bundle. IL-2 signals through the IL-2 receptor, a complex consisting of tree subunits, termed alpha, beta and gamma. The IL-2 R gamma is shared by cytokine receptors of all members of cytokine family. Mature mouse IL2 shares 56% and 73% aa sequence identity with human and rat IL2, respectively. IL-2 is produced by CD4+ T cell, CD8+ T cells, gamma δ T cells, B cells, dendritic cells and eosinophils, and plays a vital role in key function of the immune system, tolerance and immunity, primarily via its potent stimulatory activity for T cells.Thus, IL2 may be a key cytokine in the natural suppression of autoimmunity.

生物活性
- Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The EC50 this effect is less than 0.2ng/ml.
电泳(SDS-PAGE)
- 1μg (R: reducing condition, N: non-reducing condition).
反相高效液相色谱(RP-HPLC)
- Purity > 98.9% determined by RP-HPLC
ELISA
- Immobilized IL-2 Protein, Mouse (Cat. No. UA040171) at 2μg/mL (100μL/well) can bind Mouse Soluble IL2R alpha/CD25 Protein, His tag with EC50 of 7.9-15.5 ng/mL.

引用文献(1)

A novel FTO-targeting nanodrug induces disulfidptosis and ameliorates the suppressive tumor immune environment to treat uveal melanoma
Hao Tian; Hongpei Deng; Xinlong Liu; Chang Liu; Chuan Zhang; Kam W. Leong; Jing Ruan; Xianqun Fan

Biomaterials. 2025 Feb 12 ; 0142-9612

影响因子： 12.8

货号：UA040171产品名称：IL-2 Protein, Mouse