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RANTES/CCL5 Protein, Human

C-C motif chemokine 5,EoCP,Eosinophil chemotactic cytokine,SIS-delta,Small-inducible cytokine A5,T cell-specific protein P228 (TCP228),T-cell-specific protein RANTES

价格 ￥945.00 （ 供应商现货 ： 3-5个工作日）
货号 UA040168
规格
数量
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产品介绍

产品介绍 引用文献(1) 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    C-C motif chemokine 5; EoCP; Eosinophil chemotactic cytokine; SIS-delta; Small-inducible cytokine A5; T cell-specific protein P228 (TCP228); T-cell-specific protein RANTES;

  • Accession

    P13501

  • 表达序列

    Ser24-Ser91

  • 表达宿主

    E.coli

  • 分子量

    10 kDa (Reducing)

  • 纯度

    >95% by SDS-PAGE.

  • 内毒素含量

    <0.1EU/μg

  • 活性

    EC50 = 2.79-4.81 ng/mL

  • 标记

    Unconjugated

  • 标签

    No Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    20mM Tris, 800mM NaCl, pH8.0

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    ·3 months, -20 to -80℃ under sterile conditions after reconstitution.
    ·1 week, 2 to 8℃ under sterile conditions after reconstitution.
    ·Please avoid repeated freeze-thaw cycles.

  • 文献引用

    Annu Rev Immunol. 2014:32:659-702.
    Genes Dis. 2022 Jan;9(1):12-27.

背景介绍

  • RANTES/CCL5 (Regulated upon Activation, Normal T-cell Expressed and Secreted) is a member of the CC chemokine family first identified by subtractive hybridization as a T-cell-specific transcript. It is an 8 kDa protein secreted by platelets, T cells, endothelial cells, fibroblasts, epithelial cells, and many tumor types. RANTES exerts its biological effects through binding to the G-protein-coupled receptors CCR1, CCR3, CCR4 and CCR5, triggering intracellular calcium flux and directional migration of monocytes, memory T cells, eosinophils, basophils and natural killer cells. In addition to its chemotactic activity, RANTES can oligomerise on glycosaminoglycans of the endothelial surface, forming immobilised gradients that guide leukocyte extravasation. Proteolytic removal of its two N-terminal amino acids by CD26/DPPIV converts RANTES into a potent receptor antagonist that blocks HIV-1 entry through CCR5.

  • 电泳

    • 2μg (R: reducing condition, N: non-reducing condition).

  • ELISA

    • Immobilized RANTES/CCL5 Protein, Human (Cat. No. UA040168) at 5.0μg/mL (100μL/well) can bind PF-4/CXCL4 Protein, Human hFc Tag with EC50 of 5.62-9.32 ng/mL.

    • Immobilized RANTES/CCL5 Protein, Human (Cat. No. UA040168) at 5.0μg/mL (100μL/well) can bind PF-4/CXCL4 Protein, Human His Tag with EC50 of 2.79-4.81 ng/mL.

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