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物种
Human分子别名
Insulin-Like Growth Factor II,Somatomedin-A,IGF2Accession
P01344-1表达序列
Ala25-Glu91表达宿主
E.coli分子量
8kDa (Reducing)纯度
＞95% by SDS-PAGE内毒素含量
<0.1EU/μg活性
< 5 ng/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20mM Tris, 300mM NaCl, pH8.0溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
James Brown 1, E Yvonne Jones, Briony E Forbes Keeping IGF-II under control: lessons from the IGF-II-IGF2R crystal structure. Trends Biochem Sci. 2009 Dec;34(12):612-9. Epub 2009 Sep 30.
Insulin-like growth factor-II (IGF-II) is a key regulator of cell growth, survival, migration and differentiation. Its pivotal role in these processes requires tight regulation of both expression and activity. The type 1 IGF receptor tyrosine kinase (IGF-1R) mediates IGF-II actions, and a family of six high affinity IGF binding proteins (IGFBPs) regulates IGF-II circulating half-life and its availability to bind IGF-1R.
生物活性
Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. The ED50 for this effect is less than 5ng/ml
电泳
2μg (R: reducing condition, N: non-reducing condition).
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