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产品规格
物种
Mouse分子别名
Ciliary neurotrophic factorAccession
P51642表达序列
Ala2-Met198表达宿主
E.coli分子量
23kDa纯度
＞95% by SDS-PAGE内毒素含量
<0.1EU/μg标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
1xPBS, 2mM TCEP, pH 7.4溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
Prog Retin Eye Res. 2012 Mar;31(2):136-51.Epub 2011 Dec 10. Eur Cytokine Netw. 2004 Jul-Sep;15(3):255-62.
背景介绍
Ciliary neurotrophic factor (CNTF) is one of the most studied neurotrophic factors for neuroprotection of the retina. It is a neuroprotective cytokine initially identified in chick embryo. It has been evaluated for the treatment of neurodegenerative diseases. CNTF also acts on non-neuronal cells such as oligodendrocytes, astrocytes, adipocytes and skeletal muscles cells.
生物活性
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The EC50 for this effect is less than 30ng/ml.
电泳
1μg (R: reducing condition, N: non-reducing condition).
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