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物种
Mouse分子别名
Interleukin-6蛋白, IL-6蛋白;Interleukin-6, IL-6, B-Cell Hybridoma Growth Factor, Interleukin HP-1, Il6, Il-6Accession
P08505表达序列
Phe25-Thr211
表达宿主
E.coli分子量
22kDa
纯度
＞95% by SDS-PAGE&RP-HPLC内毒素含量
<0.1EU/μg标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
1x PBS, pH 7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1. Cold Spring Harb Perspect Biol. 2014 Sep 4;6(10):a016295.
2. Cold Spring Harb Perspect Biol. 2018 Aug 1;10(8):a028456.
3. Biochem J. 2003 Aug 15;374(Pt 1):1-20.
Interleukin-6 (IL-6) is a pro-inflammatory cytokine that also has an important role in immunity. Mouse IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 in regulating the acute phase response. Mouse IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 has a wide variety of biological functions: it plays an essential role in the final differentiation of B-cells into Ig-secreting cells, it induces myeloma and plasmacytoma growth, nerve cells differentiation in hepatocytes, and acute phase reactants.
生物活性
Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The EC50 for this effect is less than 0.1ng/ml.
电泳
反相高效液相色谱(RP-HPLC)
ELISA
Immobilized IL-6 Protein, Mouse (Cat. No. UA040155) at 2.0μg/mL (100μL/well) can bind IL-6R alpha/CD126 Fc Chimera Protein, Mouse (Cat. No.UA011252) with EC50 of 4.66-7.37ng/mL.
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