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物种
Human分子别名
Platelet-Derived Growth Factor-AA, Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGFAccession
P04085表达序列
Ser87-Thr211
表达宿主
E.coli分子量
18kDa (Reducing), 32kDa (Non-reducing)
纯度
＞95% by SDS-PAGE内毒素含量
<0.1EU/μg活性
< 3 ng/ml标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20mM Tris, 100mM NaCl, pH8.0
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
Souza P, Kuliszewski M, Wang J, Tseu I, Tanswell AK, Post M. PDGF-AA and its receptor influence early lung branching via an epithelial-mesenchymal interaction. Development. 1995 Aug;121(8):2559-67. Demaria M, Ohtani N, Youssef SA, Rodier F, Toussaint W, Mitchell JR, Laberge RM, Vijg J, Van Steeg H, Dollé ME, Hoeijmakers JH, de Bruin A, Hara E, Campisi J. An essential role for senescent cells in optimal wound healing through secretion of PDGF-AA. Dev Cell. 2014 Dec 22;31(6):722-33.
Platelet-derived growth factor (PDGF) is a family of three disulfide-linked glycoprotein dimers of M.W. 29kDa-32kDa that arise from astochastic assembly of the homologous subunits, A-chain and B-chain, yielding the heterodimer PDGF-AB and homodimers PDGF-AA and PDGF-BB. PDGF-AA and its receptor represent an important epithelial-mesenchymal interaction which plays a critical role in early lung branching morphogenesis.
生物活性
Measured in a cell proliferation assay using NIH-3T3 mouse fibroblast cells, the EC50 for this effect is less than 30ng/ml.
电泳
1μg (R: reducing condition, N: non-reducing condition).
ELISA
Immobilized PDGF-AA, Human(Cat. No. UA040146) at 2.0μg/mL (100μL/well) can bind PDGF R alpha/PDGFRA Fc Chimera, Human(Cat. No. UA010718) with EC50 of 11.40-14.74 ng/mL.
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