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产品规格
物种
Rhesus macaque分子别名
TNF-alpha,Tumor Necrosis Factor,Cachectin,Tumor Necrosis Factor Ligand Superfamily Member 2,TNF-a,TNF,TNFA,TNFSF2Accession
P48094表达序列
Val77-Leu233表达宿主
E.coli分子量
17.3kDa纯度
>95% by SDS-PAGE内毒素含量
<0.1EU/μg标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
PBS, 2mM TCEP, pH 7.4溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1.Microsc Res Tech. 2000 Aug 1;50(3):184-95.
2.Methods Mol Biol. 2021;2248:271-279.
背景介绍
Tumour Necrosis Factor α (TNFα), is an inflammatory cytokine produced by macrophages/monocytes during acute inflammation and is responsible for a diverse range of signalling events within cells, leading to necrosis or apoptosis. The protein is also important for resistance to infection and cancers. In view of these facts, TNF-α has been recommended as an important target for discovering drugs for autoimmune and inflammatory diseases and cancer.
生物活性
Measured in a cell proliferation assay using L-929 mouse fibrosarcoma cells, the EC50 for this effect is less than 30pg/ml.
电泳
1μg (R: reducing condition, N: non-reducing condition).
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