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物种
Mouse分子别名
M-CSF,CSF-1,Macrophage Colony Stimulating FactorAccession
P07141-1表达序列
Lys33-Pro187
表达宿主
E.coli分子量
18.2 kDa (Reducing)
纯度
＞95% by SDS-PAGE内毒素含量
<0.1EU/μg活性
<2ng/ml标记
Unconjugated标签
No Tag性状
Lyophilized powder缓冲体系
20mM Tris，200mM NaCl,pH8.0
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1. Cosman D, Wignall J, Anderson D, et al. 1988. Behring Inst Mitt: 15-26.
Macrophage-Colony Stimulating Factor (M-CSF), also known as Colony Stimulating Factor-1 (CSF-1), is a hematopoietic growth factor. It can stimulate the survival, proliferation and differentiation of mononuclear phagocytes, in addition to the spreading and motility of macrophages. M-CSF is mainly produced by monocytes, macrophages, fibroblasts, and endothelial cells. M-CSF interaction with its receptor, c-fms, has been implicated in the growth, invasion, and metastasis of several diseases, including breast and endometrial cancers.
生物活性
Measured in a cell proliferation assay using Murine M-NFS-60 cells, the EC50 for this effect is less than 10ng/ml.
电泳
1μg (R: reducing condition, N: non-reducing condition).
ELISA
Immobilized M-CSF Protein, Mouse (Cat. No. UA040128) at 2.0μg/mL (100μL/well) can bind M-CSFR mFc Chimera, Mouse(Cat. No. UA010188) with EC50 of 5.52-8.06 ng/ mL.
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