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物种
Human分子别名
Interleukin-7Accession
P13232表达序列
Asp26-His177 with C-terminal 6*His Tag
DCDIEGKDGKQYESVLMVSIDQLLDSMKEIGSNCLNNEFNFFKRHICDANKEGMFLFRAARKLRQFLKMNSTGDFDLHLLKVSEGTTILLNCTGQVKGRKPAALGEAQPTKSLEENKSLKEQKKLNDLCFLKRLLQEIKTCWNKILMGTKEHHHHHHH
表达宿主
HEK293分子量
18-30 kDa (Reducing)
纯度
＞95% by SDS-PAGE内毒素含量
<1EU/mg标记
Unconjugated标签
His Tag性状
Lyophilized Powder缓冲体系
10 mM PBS, pH 7.4.
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1. Proc Natl Acad Sci U S A. 1989 Jan;86(1):302-6.
2. J Biol Chem. 1997 Dec 26;272(52):32995-3000.
3. J Immunol. 1995 Jan 1;154(1):58-67.
Interleukin 7（IL-7）is a mature protein of 177 amino acids with a signal sequence of 25 amino acids and a calculated mass of 17.4kDa. Recombinant human IL-7 stimulated the proliferation of murine pre-B cells and was active on cells harvested from human bone marrow that are enriched for B-lineage progenitor cells.
IL-7 is a proteinaceous biological response modifier that has a bioactive tertiary structure dependent on disulfide bond formation.
IL-7-stimulated pro-B cells partially differentiated into a pre-B cell population, on the basis of the loss of CD34 and terminal deoxynucleotidyl (TdT) expression, and the acquisition of cytoplasmic mu heavy chains.
生物活性
Measured in a cell proliferation assay using 2E8 cells. The EC50 for this effect is less than 1.5ng/ml.
电泳
2μg (R: reducing conditions, N: non-reducing conditions).
SPR
Protein A Chip captured IL-7R alpha/CD127 Fc Chimera Protein, Human(Cat. No. UA010892), can bind IL-7 Protein, Human (Cat. No. UA040118) with an affinity constant of 2.68nM as determined in SPR assay.
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