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物种
Rat抗原名称
IGF-1分子别名
Insulin-Like Growth Factor I,IGFI,IGF1,IGF-1Accession
P08025表达序列
Gly49-Ala118表达宿主
E.coli分子量
8kDa (Reducing)纯度
>95% by SDS-PAGE内毒素含量
<0.1EU/μg标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20mM NaAC, pH5.0溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1. Bartlett WP, Li XS, Williams M. 1992. Brain Res Mol Brain Res, 12: 285-91.
Insulin-like growth factor I (IGF-I) belongs to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGFs are generated by proteolytic processing of inactive precursor protein containing N-terminal and C-terminal propeptide regions. IGF-I is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. The production of IGF-I is stimulated by growth hormone (GH) and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5B. IGF-I binds IGF-1R, IGF-2R, and the insulin receptor and plays a key role in cell cycle progression, cell proliferation and tumor progression. Accession#: P08025 https://www.uniprot.org/uniprotkb/ P08025 /entry
生物活性
- Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. The EC50 for this effect is less than 2ng/ml.
电泳
- 2μg (R: reducing condition, N: non-reducing condition).
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