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物种
Human分子别名
IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion;Accession
P60568表达序列
Ala21-Thr153
表达宿主
E.coli分子量
15±2kD (Reducing)
纯度
>95% by SDS-PAGE and HPLC标记
Unconjugated标签
No Tag缓冲体系
PBS, 5% trehalose pH7.4
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.文献引用
1. J Exp Med. 2020 Jan 6;217(1): e20191247.
2. Nat Rev Rheumatol. 2021 Dec;17(12):749-766.
3. Dev Comp Immunol. 2023 Apr:141:104645.
| 项目 | 标准 |
|---|---|
| 性状（冻干粉、液体） | Lyophilized Powder |
| 内毒素 | ≤10 EU/mg |
| 宿主蛋白残留 | ＜100ppm |
| 宿主DNA残留 | ＜100ppb |
| 支原体 | 阴性 |
| 无菌 | 符合 |
| pH值 | 7.1~7.7 |
| 蛋白含量 | 90%~120% |
| SDS-PAGE分子量 | 15±2kD（还原） |
| SDS-PAGE纯度 | ≥95%（非还原） |
| RP-HPLC纯度 | ≥95% |
| SEC-HPLC纯度 | ≥95% |
| 细胞活性 | ＞1.00 x 10^7 IU/mg |
Interleukin-2 (IL-2) is an interleukin, a type of cytokine immune system signaling molecule, which is a leukocytotropic hormone that is instrumental in the body natural response to microbial infection and in discriminating between foreign (non-self) and self. IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes, the cells that are responsible for immunity. Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. IL-2 is also necessary during T cell development in the thymus for the maturation of a unique subset of T cells that are termed regulatory T cells (T-Regs). After exiting from the thymus, T-Regs function to prevent other T cells from recognizing and reacting against "self-antigens", which could result in "autoimmunity". T-Regs do this by preventing the responding cells from producing IL-2. Thus, IL-2 is required to discriminate between self and non-self, another one of the unique characteristics of the immune system.
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