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物种
Human, Human抗原名称
TSLP分子别名
TSLP; Thymic stromal lymphopoietinAccession
Q969D9表达序列
Tyr29-Gln159
表达宿主
E.coli分子量
13-15kDa (Reducing)
纯度
>95% by SDS-PAGE内毒素含量
<0.1EU/μg标记
Unconjugated标签
No Tag性状
Lyophilized Powder缓冲体系
20mM Tris, 500mM NaCl, pH8.0
溶解方法
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
储存条件
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
文献引用
1. Luo J, Zhu Z, Zhai Y, Zeng J, Li L, Wang D, Deng F, Chang B, Zhou J, Sun L. The Role of TSLP in Atopic Dermatitis: From Pathogenetic Molecule to Therapeutical Target. Mediators Inflamm. 2023 Apr 15;2023:7697699. 2. 2.Lu H, Wu X, Peng Y, Sun R, Nie Y, Li J, Wang M, Luo Y, Peng L, Fei Y, Zhou J, Zhang W, Zeng X. TSLP promoting B cell proliferation and polarizing follicular helper T cell as a therapeutic target in IgG4-related disease. J Transl Med. 2022 Sep 8;20(1):414.
Thymic stromal lymphopoietin (TSLP) acts as a hemopoietic cytokine, proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. It is positively associated with AD deterioration. Mainly secreted by keratinocytes, TSLP interacts with multiple immune cells (including dendritic cells, T cells, and mast cells), following induction of Th2-oriented immune response during the pathogenesis of AD.
电泳
- 2μg (R: reducing conditions, N: non-reducing conditions).
ELISA
Measured by its binding ability in a functional ELISA. When TSLP, Human is immobilized 10µg/mL (100µl/well), TSLPR His Tag，Human(Cat.No: UA010490) binds with an EC50 of 1.5-2.0μg/ml.
Immobilized TSLP Protein, Human (Cat. No. UA040102) at 2.0μg/mL (100μL/well) can bind V6 Anti-Human TSLP (Tezepelumab) with EC50 of 1.35-1.99 ng/mL.
Immobilized TSLP, Human (Cat. No. UA040102) at 2.0μg/mL (100μL/well) can bind TSLPR Fc Chimera Protein, Human (Cat. No. UA010907) with EC50 of 0.83-1.33 ng/mL.
SPR
Anti-His antibody Immobilized on CM5 Chip captured TSLPR His Tag, Human (Cat. No. UA010490), can bind TSLP, Human (Cat. No. UA040102) with an affinity constant of 0.70 nM as determined in SPR assay.
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